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. 2023 Feb 8;13:2271. doi: 10.1038/s41598-023-29558-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Secretomic and proteomic analyses of HBD2-stimulated HaCaT cells. HaCaT cell monolayers were treated with 0.5 μg/ml HBD2, or vehicle control, in serum free media for 48 h before supernatants (a,b) and cells (c) were collected and centrifuged at 3000 rpm for 5 min. Media (a,b) and dry cell pellet (c) were stored at -80 °C prior to analysis. Data representative of n = 5. Log fold changes and p values generated using LIMMA pathway³⁷. (a,b) Volcano plots generated from secretomics analysis of HBD2-stimulated HaCaT cells compared to control vehicle-treated cells. Red points indicate significant points, blue indicate nonsignificant points. (a) Data represented as a whole. (b) Data represented without O15263 data point, zoomed in to central portion of graph. (c) Volcano plot generated from proteomics analysis of HBD2-stimulated HaCaT cells compared to control vehicle-treated cells. Red points indicate significant points, blue indicate nonsignificant points.