Skip to main content
. 2023 Feb 8;13:2271. doi: 10.1038/s41598-023-29558-0

Table 1.

Secretomic analysis of HBD2-stimulated HaCaT cells.

Protein code Gene Log2 fold change p.mod Protein name
O15263 DEFB4A 10.96 0.00 Beta-defensin 4A
Q9NX55 HYPK 1.37 0.04 Huntingtin-interacting protein K
P01833 PIGR 1.28 0.03 Polymeric immunoglobulin receptor
O95861 BPNT1 1.03 0.04 3'(2'),5'-bisphosphate nucleotidase 1
P59666 DEFA1/3 1.01 0.04 Neutrophil defensin 3
Q96CX2 KCTD12 0.94 0.01 BTB/POZ domain-containing protein KCTD12
O43920 NDUFS5 0.89 0.03 NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
P42330 AKR1C3 − 1.04 0.02 Aldo–keto reductase family 1 member C3
Q92878 RAD50 − 1.07 0.01 DNA repair protein RAD50
Q15046 KARS − 1.10 0.04 Lysine–tRNA ligase
P02794 FTH1 − 1.12 0.02 Ferritin heavy chain
P81605 DCD − 1.13 0.02 Dermcidin
P14927 UQCRB − 1.16 0.04 Cytochrome b-c1 complex subunit 7
Q9NWH9 SLTM − 1.16 0.00 SAFB-like transcription modulator
P55786 NPEPPS − 1.24 0.01 Puromycin-sensitive aminopeptidase
P62841 RPS15 − 1.24 0.01 40S ribosomal protein S15
P38919 E1F4A3 − 1.36 0.05 Eukaryotic initiation factor 4A-III
P46937 YAP1 − 1.43 0.01 Transcriptional coactivator YAP1
Q15417 CNN3 − 1.72 0.01 Calponin-3
P35527 KRT9 − 2.34 0.02 Keratin, type I cytoskeletal 9

HaCaT cell monolayers were treated with 0.5 μg/ml HBD2, or vehicle control, in serum free media for 48 h before supernatants were collected, centrifuged at 3000 rpm for 5 min, and stored at -80 °C prior to analysis; n = 5 per condition. Log fold changes and p values were generated using LIMMA pathway43. Table shows proteins with the greatest differences in detection levels between control and HBD2 treated conditions, listed by fold change, largest to smallest, with Uniprot protein code, gene code, fold change, p.mod value, protein name and brief description of function. Protein descriptions made using UniProt database information46.