Table 2.

Proteomic analysis of HBD2-stimulated HaCaT cells.

Protein code	Gene	Log2 fold change	p.mod	Protein name
P12763	Bovine AHSG	2.02	0.03	Bovine Alpha-2-HS-glycoprotein
Q14108	SCARB2	1.53	0.01	Lysosome membrane protein 2
Q8IWA4	MFN1	1.43	0.03	Mitofusin-1
Q969N2	PIGT	1.27	0.01	GPI transamidase component PIG-T
O15263	DEFB4A	1.25	0.00	Beta-defensin 4A
P00374	DHFR	1.22	0.03	Dihydrofolate reductase
P34955	Bovine SERPINA1	1.20	0.03	Bovine Alpha-1-antiproteinase
Q8WWQ0	PHIP	1.06	0.03	PH-interacting protein
O43272	PRODH	1.03	0.03	Proline dehydrogenase 1, mitochondrial
Q9NVJ2	ARL8B	1.01	0.00	ADP-ribosylation factor-like protein 8B
Q9UNN8	PROCR	1.01	0.02	Endothelial protein C receptor
P62745	RHOB	− 1.01	0.00	Rho-related GTP-binding protein RhoB
Q9Y6M5	SLC30A1	− 1.02	0.01	Zinc transporter 1
Q8N0U4	FAM185A	− 1.14	0.00	Protein FAM185A
Q8IYD1	GSPT2	− 1.19	0.03	Eukaryotic peptide chain release factor GTP-binding subunit ERF3B
P83876	TXNL4A	− 1.27	0.00	Thioredoxin-like protein 4A
P13688	CEACAM1	− 1.27	0.02	Carcinoembryonic antigen-related cell adhesion molecule 1
P60510	PPP4C	− 1.60	0.03	Serine/threonine-protein phosphatase 4 catalytic subunit
P42212	GFP	− 2.17	0.05	Jellyfish Green fluorescent protein
Q61782	Mouse Q61782	− 3.46	0.14	Mouse Type I epidermal keratin mRNA

HaCaT cell monolayers were treated with 0.5 μg/ml HBD2, or vehicle control, in serum free media for 48 h before the cells were washed rigorously with PBS and scraped from the well surface. Cell pellets were washed an additional three times with PBS in suspension. Dry cell pellets were stored at -80 °C prior to analysis; n = 5 per condition. Log fold changes and P values generated using LIMMA pathway⁴³. Table shows proteins with the greatest differences in detection levels between control and HBD2 treated conditions, largest to smallest with Uniprot protein code, gene code, fold change, p.mod value, protein name and brief description of function. Protein descriptions made using UniProt database information⁴⁶.