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. 2023 Feb 8;9:8. doi: 10.1038/s41522-023-00374-8

Fig. 5. DCA alleviates S. aureus-induced inflammation in MMECs through the cAMP-PKA- NF-Κb/NLRP3 pathways.

Fig. 5

A–G and O. Cells were pretreated with H89 (30 μM) and MDL12330A (10 μM) for 2 h and then treated with DCA (30 μM) for an additional 2 h followed by S. aureus treatment for the next 24 h. The protein levels of NF-κB and NLRP3 pathways from the indicated groups were determined by western blotting (A). The relative intensities of p-p65, p-IκB, NLRP3, ASC, and IL-1β were determined (B–G). The relative mRNA levels of proinflammatory gene from the indicated group were detected by qPCR (O). H–N and P. Cells were pretreated with siRNA for 48 h and then treated with DCA (30 μM) and KH7 (10 μM) for an additional 2 h followed by S. aureus treatment for the next 24 h. The protein levels of NF-κB and NLRP3 pathways from the indicated groups were determined by western blotting (H) and The relative intensities of p-p65, p-IκB, NLRP3, ASC and IL-1β were determined (I–N). The relative mRNA levels of proinflammatory gene from the indicated group were detected by qPCR (P). Data are expressed as the means ± SD (B–G, I–N and O, P) and one-way ANOVA was performed, followed by Tukey’s test (B–G, I–N, and O, P). *p < 0.05, **p < 0.01, ***p < 0.001 indicate significance.