Table 1.
Monoclonal antibodies used in the present study
| Antibody | Isotype | Origin | Expression | Description |
|---|---|---|---|---|
| E4 | mIgG2bb | RA patients | Expi293F | Chimeric antibody (human variable domain + mouse constant domain). |
| L1-L12a | mIgG2bb | RA patients | Expi293F | Chimeric antibodies (human variable domain + mouse constant domain identical to E4). |
|
E4-mutant (E4m) |
mIgG2bb | RA patients | Expi293F | E4 antibody with two mutations (W48M & S51A) abolishing citrulline binding. |
| E4NG | mIgG2bb | RA patients | Expi293F | E4 antibody with mutations in Fab glycosylation sites that change the antibody to non-glycosylated form. |
| M2139 | mIgG2b | Mouse | Hybridoma | Murine arthritogenic antibody specific to the J1 epitope on collagen type II (COL2). |
| M2139m | mIgG2b | Mouse | Expi293F | M2139 antibody with a point-mutation (S31R) that abolishing binding to COL2 and arthritogenicity. |
| ACC4 | mIgG1 | Mouse | Hybridoma | Murine arthritis-enhancing ACPA specific to citrullinated C1 alpha-chain on COL2. |
| ACC1 | mIgG2a | Mouse | Hybridoma | Murine arthritis-inducing ACPA specific to citrullinated/native triple helical epitopes on COL2. |
| E4 | hIgG1 | RA patient | Expi293F | E4 antibody with human IgG1 isotype. |
| M2139 | hIgG1 | Mouse | Expi293F | M2139 antibody with human IgG1 isotype. |
aL1-L12 are the simplified labels for clones originally entitled as 2D11, 1F2, 1G8, 2C11, 1C7, 2D7, 2F10, 1D10, C3, F6, 2E2 and 3C5, respectively.
bChimeric antibodies with identical mouse constant domain.