Table 2.
Experiment design to quantify the efficacy of the rHVT-H9 in the transmission of H9N2 AIV in chickens with PTAs.
| Group | Vaccination | Subgroup | Challenge/dose | Samplings/duration |
|---|---|---|---|---|
| 1 | rHVT-H9 | Sacrificed (n = 3) | None | Feather, spleen and lavage liquids from the nasal cavity and trachea |
| Inoculated (n = 5) | Intranasal/H514, 106EID50 | Oronasal and cloaca swabs/ 14 days | ||
| Contact (n = 5) | Contact | Oronasal and cloaca swabs/ 14 days | ||
| 2 | H9N2 IWV | Sacrificed (n = 3) | None | Feather, spleen and lavage liquids from the nasal cavity and trachea |
| Inoculated (n = 5) | Intranasal/H514, 106EID50 | Oronasal and cloaca swabs/ 14 days | ||
| Contact (n = 5) | Contact | Oronasal and cloaca swabs/ 14 days | ||
| 3 | PBS | Sacrificed (n = 3) | None | Feather, spleen and lavage liquids from the nasal cavity and trachea |
| Inoculated (n = 5) | Intranasal/H514, 106EID50 | Oronasal and cloaca swabs/ 14 days | ||
| Contact (n = 5) | Contact | Oronasal and cloaca swabs/ 14 days |