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. 2021 Dec 28;54(1):137–147. doi: 10.3724/abbs.2021014

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© The Author(s) 2021.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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S100A6 negatively regulates the osteogenic differentiation of osteosarcoma cellsOsteosarcoma cells were cultured in the osteogenic induction medium for 14 days after transfection with different plasmids. The expressions of S100A6, OPN, and OCN in (A) MG63 and (B) 143B cells; left: protein level, right: mRNA level. The matrix mineralization of (C) MG63 and (D) 143B cells was detected by Alizarin Red Staining. ALP staining and ALP activity of (E) MG63 and (F) 143B cells; left: ALP staining, right: ALP activity. *P<0.05 and **P<0.01.