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. 2023 Jan 26;17:974197. doi: 10.3389/fnins.2023.974197

FIGURE 2.

FIGURE 2

Quantitative analysis of the anatomical relationship between afferent axons and glomeruli in TRPV1:Ai14 and wildtype C57BL/6J mice. (A) Prevalence of calcitonin gene related peptide (CGRP)+ afferent axons near glomeruli in TRPV1:Ai14 mice (n = 4), presented by glomerular category based on depth within the cortex. Glomeruli at all depths were found to have similar frequencies of anatomical interactions with CGRP+ axons in TRPV1:Ai14 mice (Superficial: 40.78 ± 9.50%, Midcortical: 38.09 ± 6.29%, Juxtamedullary: 57.74 ± 7.70%; total number of evaluated glomeruli: 1346, 1 slice per animal). (B) Prevalence of tdTomato+ afferent axons near glomeruli in TRPV1:Ai14 mice (n = 5), presented by glomerular category based on depth within the cortex. Glomeruli at all depth categories were found to have similar frequencies of anatomical interactions with tdTomato+ axons in TRPV1:Ai14 mice (Superficial: 55.14 ± 7.06%, Midcortical: 54.22 ± 5.59%, Juxtamedullary: 72.40 ± 4.98%; total number of evaluated glomeruli: 1476, 1 slice per animal). (C) Prevalence of CGRP+ afferent axons near glomeruli in wildtype C57bl6/j mice (n = 6), presented by glomerular category based on depth within the cortex. Deeper glomeruli had closely apposed CGRP+ axon bundles more frequently than more superficial glomeruli (Superficial: 33.00 ± 3.11%, Midcortical: 41.22 ± 3.05%, Juxtamedullary: 60.57 ± 2.90%; total number of evaluated glomeruli: 3532, 1 slice per animal). (D–F) Examples of anatomical relationships between CGRP+ axons and glomeruli in TRPV1:Ai14 mice (D), tdTomato+ axons and glomeruli in TRPV1:Ai14 mice (E), and CGRP+ axons and glomeruli in C57bl6/j wildtype mice (F) as presented while scoring individual glomeruli (scale bars: 50 μm). (Mean ± SEM, *p < 0.05, **p < 0.01 by Repeated Measures One-Way ANOVA with multiple comparisons).