Skip to main content
. 2023 Jan 26;17:974197. doi: 10.3389/fnins.2023.974197

FIGURE 3.

FIGURE 3

Analysis of close appositions between glomeruli and afferent axons. (A) Maximum intensity projection of 136 optical sections, collected 0.65 μm apart, total volume 88.4 μm. (B–D) Maximum intensity projections of subvolumes of A depict close appositions between calcitonin gene related peptide (CGRP)+ axons and nephrin labeling. Optical section numbers included in each projection are indicated above the scale bars. CGRP labeling does not overlap with the halo-like fluorescence surrounding nephrin labeling (faint green), suggesting that the axons do not penetrate below Bowman’s capsule, but are immediately apposed to it. Panels (B,C) illustrate an approximately 11.2 μm-long segment of CGRP labeling located 0.4–1.3 μm from the halo-like fluorescence surrounding nephrin labeling. (E) Distribution of the minimum distance between nephrin and CGRP labeling in high-resolution images of 42 glomeruli (n = 7 animals, 6 glomeruli per animal). (F) Minimum distance between nephrin and CGRP labeling by glomerular category, displayed as overlay [n = 7 animals, 2 glomeruli per category each (42 total)]. Scale bars: (A) 25 μm, (B–D) 10 μm.