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. 2021 Dec 23;54(2):220–231. doi: 10.3724/abbs.2021023

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© The Author(s) 2021.

This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/).

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CPT1A overexpression decreases the cholesterol absorption and intracellular lipid accumulation(A,B) Contents of TC and TG in CPT1A-overexpressing Caki-1, 786O cell and their corresponding normal control cell lines were detected by ELISA kits. (C) Cholesterol deposition in CPT1A-overexpressing Caki-1 and 786O cells and their corresponding normal control cells. Scale bar = 50 μm. (D) Cholesterol absorption between CPT1A-overexpressing Caki-1 and 786O and their corresponding normal control cells detected by Flow cytometry. (E) The protein expressions of CD36, ABCG1, SRB1, LOX1 in CPT1A-overexpressing Caki-1, and 786O cell and their corresponding normal control cell lines determined by western blot analysis. Values are shown as the mean±SD. ***P<0.001 (Student’s t-test).