Reagent	Final concentration	Amount
Tris (50 mM pH 8)	~50 mM	79 µL
Atto azide Alexa 647 (6 mM)	60 µM	1 µL
CuSO 4 (40 mM)	4 mM	10 µL
Ascorbic acid (20 mg/mL)*	2 mg/mL	10 µL
Total	n/a	100 µL