Reagent	Final concentration	Amount
MgCl2 (1 M)	10 mM	1 mL
MES-NaOH (0.5 M, pH 5.6)	10 mM	2 mL
Acetosyringone (100 mM) H2O	0.15 mM n/a	1.5 mL 95.5 mL
Total	n/a	100 mL