Table 3. Therapeutics and dressings.

Step	Procedure	Troubleshooting
1	Injecting therapeutic (e.g., cells, pharmacologic) or no treatment (if using hydrogels, skip to step 2)
1a	Inject therapeutic or vehicle control around the wound edge.
1b	Wrap the wounds with an initial layer of Telfa.
1c	Skip to step 3.
2	Hydrogel therapy (Figure 2A)
2a	Apply piece of circular hydrogel onto the wound (with or without therapeutic imbued) (Figure 2B).	Hydrogel should be cut out so that it has the same measurements as the excisional wound.
2b	Wrap the wounds with an initial layer of Tegaderm.	Initial layer keeps hydrogel protected, moist, and pressed against wound to maximize delivery.
2c	Next, cover the wound with Telfa dressing.
3	Cut Tegaderm sheets in half and use the halves to wrap around the mice (Figure 1B).
4	Monitor mouse until it is fully awake.
5	Place in cage—single housing is necessary now.