Table 4. Monitoring and wound measurements.

Step	Procedure	Troubleshooting
1	Monitor mouse daily.
2	Every other day: measure wound using the following steps.
3	Anesthetize mouse as described in section B.	Monitor mouse carefully and take out of anesthesia box immediately when sufficient level of anesthesia has been reached. Exposure to high flow rates of isoflurane for too long can cause death.
4	Carefully remove wound dressing (Tegaderm and Telfa).	Be sure to remove the dressings carefully so as to not disturb the wound bed or tear off splints and sutures.
5	Take a picture of wound for analysis as described in Step B17.
6	Check if splints are securely fixed to skin; otherwise, the wound will contract and cannot be used for analysis anymore.
7	If splint is not securely fixated to skin, reapply Vetbond and 6-0 interrupted suture(s).	Examples of damaged splints and explanation on how to deal with them are shown in Figure 4A–F.
8	Re-apply dressings and/or therapeutics as described in section C.