Table 5. Explant and analysis.

Step	Procedure	Troubleshooting
1	Anesthetize mouse as described in section B.
2	Take off wound dressing.
3	Take a picture of wound for analysis as described in Step B17.
4	Mark area of excisional wound with a surgical skin marker.
5	Remove splints from underlying skin by carefully cutting sutures and peeling off.	Take care not to disturb wounded skin as this can lead to tissue damage.
6	Euthanize mouse using neck dislocation while still under anesthesia.
7	Immediately after euthanizing, excise skin within marked area of the wound.
8	Excised tissue can now be further processed for desired analysis techniques. Further data analysis techniques of the tissue can include: Histological staining (H&E, Trichrome, etc.) Immunofluorescent staining for proteins Single-cell RNA sequencing Mechanical testing