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. 2021 Dec 23;54(2):271–274. doi: 10.3724/abbs.2021024

Table1 Sequences of primers used in qPCR

Gene

GenBank acce-ssion No.

Primer sequence (5′→3′)

IL-6

NC_000071

Forward: TGCCTTCTTGGGACTGATGC

Reverse: GCAAGTGCATCATCGTTGTTC

IL-12

NM_001303244

Forward: GGAAGAGTCCCCCAAAAGCT

Reverse: CAGCAAAGGTGTCATGATGAACTT

IL-18

NM_008360

Forward: ACCAAGTTCTCTTCGTTGAC

Reverse: CTTCACAGAGAGGGTCACAG

IL-1β

NM_008361

Forward: AGGAGAACCAAGCAACGACA

Reverse: CTCTGCTTGTGAGGTGCTGA

TNF-α

NM_013693

Forward: GACGTGGAACTGGCAGAAGA

Reverse: GGCTACAGGCTTGTCACTCG

NOD1

NM_172729.3

Forward: GATTGGAGACGAAGGGGCAA

Reverse: CGTCTGGTTCACTCTCAGCA

NOD2

NM_145857.2

Forward: GCCAGTACGAGTGTGAGGAG

Reverse: GCGAGACTGAGTCAACACCA

NLRP1

NC_000077

Forward: ATAAACAAGCCACCCCCAGT

Reverse: TGTGCCCAATGTCGATCTCA

NLRP3

NM_145827.4

Forward: AGCCAGAGTGGAATGACACG

Reverse: CGTGTAGCGACTGTTGAGGT

NLRC4

NM_001033367

Forward: GCTCAGTCCTCAGAACCTGC

Reverse: ACCCAAGCTGTCAATCAGACC

ACTIN

NM_007393

Forward: GCCATGTACGTAGCCATCCA

Reverse: ACGCACGATTTCCCTCTCAG

Caspase-1

NC_000075.7

Forward: AACCACTCGTACACGTCTTGC

Reverse: ATCCTCCAGCAGCAACTTCA