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. 2023 Jan 26;14:1086280. doi: 10.3389/fimmu.2023.1086280

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Copyright © 2023 Zhang, Cao, Luo, Zhang, Wang, Dai, Wu, Liu, Xie, Cheng and Cheng

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RUNX1/CD44 axis mediates proliferation and migration of glioma. (A) Correlation between CD44 and RUNX1, PD-L1 in the TCGA dataset. (B) qPCR assay for CD44 in shRNA-transfected U251 cells. (C) CCK8 assay for four groups of U251 cells. (D) Western blotting assay for RUNX1, CD44, and PD-L1 in shRNA-transfected U251 cells. (E) Statistical analysis of western blotting assay for RUNX1, CD44, and PD-L1. (F) Clone formation assay for four groups of U251 cells. (G) Transwell assay for four groups of U251 cells. NS, Not Statistically Significant; *, P< 0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001.