Figure 5.

Caspase-11 significantly contributes to maintaining dual fuel bioenergetics- glycolysis and OXPHOS in macrophages potentially for cholesterol synthesis and trained immunity. Bone marrow macrophages were isolated from 3 WT and 3 Casp11^–/– mice and treated with palmitic acid (500 µM) for 8 hours then pulled for seahorse analysis. (A) Seahorse XF96 Extracellular Flux Analyzer to measure extracellular acidification rate (glycolysis) of Casp11^–/– vs WT BMDMs in palmitic acid supplemented medium. (B) Seahorse mitochondrial function assay of Casp11^–/– vs WT BMDMs in palmitic acid supplemented medium.*P < 0.0, ****p < 0.0001.