Table 1.
Primer sequence for various genes.
| Gene | Primer Sequence | Annealing temperature | |
|---|---|---|---|
| 16srRNA | GTGGCGAACGGGTGAGTAAC ATGCATCCCGTGGTCCTATC |
(Forward) (Reverse) |
60°C |
| hspX | CACCACCCTTCCCGTTCAG TGGACCGGATCTGAATGTGC |
(Forward) (Reverse) |
56°C |
| tgs1 | TCGTTAATGCTGCCCAACCT CCGAATTGTCTCTGTCCCCC |
(Forward) (Reverse) |
56°C |
| tgs3 | GACATCACCTACCACGTCCG TACATCTCCCACAATGGCCG |
(Forward) (Reverse) |
64°C |
| tgs5 | GATGGGCCAGAAGATGGACC CTTGGTAAACAGCAGCACGG |
(Forward) (Reverse) |
53°C |
| mmp1 (Human) | ATGCACAGCTTTCCTCCACTG CAGCCCAAAGAATTCCTGCATT |
(Forward) (Reverse) |
57°C |
| mmp2 (Human) | AACTACGATGATGACCGCAA CTCCTGAATGCCCTTGATGT |
(Forward) (Reverse) |
54°C |
| mmp9 (Human) | GCCACTACTGTGCCTTTGAG AGAATCGCCAGTACTTCCCA |
(Forward) (Reverse) |
58°C |
| β-actin (Human) | AGAGCCTCGCCTTTGCCGATC CCCACCATCACGCCCTGGTGC |
(Forward) (Reverse) |
64°C |
| mmp1 (Mouse) | ACTACAACTGACAACCCAAGAAAG AGGAGATGCCTAGAATCACAGT |
(Forward) (Reverse) |
62°C |
| mmp2 (Mouse) | AACGGTCGGGAATACAGCAG AAACAAGGCTTCATGGGGGC |
(Forward) (Reverse) |
63°C |
| mmp9 (Mouse) | CTCTCCTGGCTTTCGGCTG TCCGTGAGGTTGGAGGTTTTC |
(Forward) (Reverse) |
63°C |
| gapdh (Mouse) | AGCTTGTCATCAACGGGAAG TTTGATGTTAGTGGGGTCTCG |
(Forward) (Reverse) |
60°C |