Figure3 .

LIMT is negatively correlated with miR-665 expression(A) StarBase V3.0 was used to predict the potential binding site for miR-665 in LIMT. (B) Relative luciferase activity of HCC cells co-transfected with WT-LIMT or Mut-LIMT and NC, or miR-665 mimics. *P<0.05. (C) The levels of miR-665 and EMT-related mRNAs were detected in HCC tumor tissues and matched adjacent tissues. The correlation between LIMT and miR-665 (**P<0.01 and ***P<0.001). (D) The level of miR-665 in HCC cells treating with LIMT siRNA or the LIMT overexpressing plasmid was determined by qRT-PCR. **P<0.01 and ***P<0.001. (E) LIMT expression was detected after incubation with miR-665 mimics or inhibitors. **P<0.01 and ***P<0.001.