FIGURE 4.

Comparison of FT-IR spectra of the hydrolytic activity of N. brasiliensis on tween 80 with and without β-HFC. Culture medium (control blue line), hydrolyzate of 7 days of N. brasiliensis growth (orange line), hydrolyzate of growth of 7 days of N. brasiliensis + β-HPC (red line). A decrease in the alcohol group (O–H) is observed, as well as the signals of alkenes (= C-H), alkanes (C–H), and carboxylic acids (COO). In contrast, more intense signals are detected mainly from ethers (C–O–C), both groups of signals, when accumulating and intensifying the signal, also indicate an increase in the utilization of Tween 80 when N. brasiliensis + β-HPC is incubated.