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. 2023 Jan 26;14:1089156. doi: 10.3389/fmicb.2023.1089156

FIGURE 6.

FIGURE 6

Interpolation of the hydrolysis of N. brasiliensis on substrates within the calibration curve. In the presence and absence of β-HPC with different standard concentrations from the calibration curves (cc) of each substrate, using the SCiDAVis program. (A) Casein spectra (cc), a transmittance value tending to 100 indicates degradation of this substrate. The spectra of hydrolyzed casein (10 g) with N. brasiliensis alone (dashed fuchsia line) or N. brasiliensis + β-HPC (dotted red line) are similar to that of 1.25 g (black line), proving that hydrolysis took place. (B) The L-tyrosine spectra showed the functional groups previously described between 1500 and 400 cm–1; even when the detected signals are shorter due to dilution. The spectrum of hydrolyzed L-tyrosine by N. brasiliensis (dashed fuchsia line) showed a broader OH signal at 3650 cm–1 with a transmittance value of 75, and at 1550 cm–1, with a transmittance of 60. On the contrary, the spectrum of hydrolyzate by N. brasiliensis + β-HPC is similar to that of 1.25 g (black line) with a transmittance greater than 90, indicating almost complete hydrolysis of the substrate. (C) In the Tween 80 cc, the hydrolyzate by N. brasiliensis corresponds to that of 0.2% concentration of this substrate. However, such results indicated a higher concentration of hydrolyzed compounds, since the concentration for the identification of this substrate is 0.1%; the spectrum hydrolyzed by N. brasiliensis + β-HPC (red dotted line) resembles that of 0.025% (green line). (D) Signals in egg yolk cc spectra show higher amplitude when this substrate is diluted, and in the 3000–3500 cm–1 range, the hydrolyzed product by N, brasiliensis resembles that of 375 μL (green line) with a transmittance value of 86. The hydrolyzed lipid of N. brasiliensis + β-HPC correlates to that of 750 μL (deep blue line) with a transmittance value of 82. N. brasiliensis + β-HPC were closest to the highest concentrations. In the protein window (1800–1500 cm–1), the substrate hydrolyzed by N. brasiliensis has a transmittance value of 60, and that hydrolyzed by N. brasiliensis + β-HPC presented a transmittance value of 54; such differences were similar throughout the mix and polysaccharide windows. However, broad signals were observed at 93, 75, and 1500 μL with transmittance values of 30 and 38 in the fingerprint area. In the presence of β-HPC, a signal with a transmittance value signal of 44 was detected.