Figure 1.

MDSC response to P. brasiliensis infection. C57BL/6 mice were infected through the intratracheal route with 1 × 10⁶ P. brasiliensis yeasts. Lungs were obtained after 96 hours, 2 weeks, and 8 weeks of infection, and compared with lungs from uninfected control animals. Characterization of subpopulations was performed using specific antibodies conjugated to fluorochromes. (A) Cells were gated by FSC/SSC analysis, and populations of PMN-MDSCs (CD45⁺CD11b⁺LY6G⁺LY6C^low) and M-MDSCs (CD45⁺CD11b⁺LY6G^-LY6C^hi) were represented in frequency and absolute numbers. (B-E) Data represent three independent experiments with 3-4 mice each. For comparisons between two groups, the mean ± SEM was obtained and analyzed by the unpaired Student’s t-test. Differences were considered significant when *p<0.05; ***p<0.001 and ****p<0.0001.