Figure 3.

Immunosuppressive molecules expressed by Monocytic-like-MDSCs (M-MDSCs) in response to P. brasiliensis infection. The expression of immunosuppressive molecules by M-MDSC was evaluated in the lung infiltrating cells of P. brasiliensis-infected mice (1×10⁶ yeasts cells) by flow cytometric analysis with 96 hours, 2 weeks, and 8 weeks of infection. The expression of cell membrane molecules (A) such as CD274 (PD-L1) and B7-H4, as well as intracellular molecules (B) IDO-1, IL-10, and nitrotyrosine (NT), were evaluated in CD45⁺CD11b⁺LY6G^-LY6C^hi cells (M-MDSC). Cells were gated by FSC/SSC analysis. Data represent three independent experiments with 3-4 mice each. For comparisons between two groups, the mean ± SEM was obtained and analyzed by the unpaired Student’s t-test. Differences were considered significant when: *p<0.05; **p<0.01; ***p<0.001 and ****p<0.0001.