Figure 6.

Effect of MDSC depletion in the presence of pulmonary myeloid cells of P. brasiliensis-infected mice. The frequency and the absolute number of pulmonary myeloid cells were evaluated in the lung-infiltrating leukocytes of anti-Gr1-treated and untreated infected mice. After 24 hours, 1 week, and 7 weeks of infection a group of mice received intraperitoneal injections of anti-Gr1 antibody (clone RB6-8C5) or rat IgG2b isotype control antibody (200 µg/dose). At 96 hours, 2 weeks, and 8 weeks of infection lungs were removed and leukocytes were obtained. The cells were gated by FSC/SSC analysis (A) and dendritic cells CD45⁺CD11b⁺CD11c⁺F4/80^- (B), macrophages CD45⁺CD11b⁺F4/80⁺ (C) and neutrophils CD45⁺CD11b⁺Ly6G^hi (D) were analyzed by flow cytometry. Data represent three independent experiments with 3-4 mice each. For comparisons between two groups, the mean ± SEM was obtained and analyzed by the unpaired Student’s t-test. Differences were considered significant when: *p<0.05; **p<0.01; ***p<0.001 and ****p<0.0001.