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. 2023 Jan 26;14:1090109. doi: 10.3389/fnagi.2022.1090109

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Copyright © 2023 Cuanalo-Contreras, Schulz, Mukherjee, Park, Armijo and Soto.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Insoluble proteins accumulated during aging and senescence bind to Thioflavin T. Thioflavin T fluorescence in arbitrary unit (a.u) of the insoluble fraction of: (A) C. elegans, day 1 (D1), day 5 (D5), and day 10 (D10) of adult life, n = 3; (B) MEFs, proliferative (PD = 3) and senescent (PD = 8), n = 3; (C) Brain; (D) Heart; (E) Liver; and (F) Intestine of mouse, young (3 m.o., n = 8), adult (12 m.o., n = 8), and old (22 m.o., n = 4). One way ANOVA, Tukey post-hoc test. T-test (FEMs), ^nsp > 0.05, ^*p ≤ 0.05, ^**p ≤ 0.01, and ^***p ≤ 0.001. Average ± SEM.