Table 2.

Summary of protein pharmacokinetics expressed in terms of the lumped physiological parameters of Figure 1B: plasma metabolic clearance Cl_p, filtration fractions f₁, f₂, and lymph flows L₁ L₂. All results are scaled to a 70 kg human using an assumed plasma volume V_p = 2800 ml. For each species, the 4 parameters L₁, L₂, V₁ and V₂ are adjusted as described and assumed constant for all proteins studied in that species. Only 2 parameters are adjusted for each protein: Cl_P and f ≈f₁≈f₂. For human and baboon: V₁/V_p =1.3, V₂/V_p= 0.4. For dog and lamb: V₁/V_p =1.0, V₂/V_p= 0.3. “Kupffer” indicate liver Kupffer cells and “Sinusoidal” are liver parenchymal cells.(**A PK model with an additional liver compartment is used for recAP. *Cl_p for recAP corresponds to the liver metabolic clearance.)

Protein	Species	MW	Catabolic organ	Clp ml/m	f1	f2	L1 ml/m	L2 ml/m
Albumin	Human	66,500	Endothelium	0.167	0.734	0.722	0.87	2.00
Monoclonal Antibody	Human
Mepolizumab		149,000	Endothelium	0.132	0.367	0.361	0.87	2.00
Infliximab		149,000	Endothelium	0.23	0.367	0.361	0.87	2.00
Alkaline phosphatase	Human
recAP**		140,000	Liver	66.2*	0.367	0.361	0.87	2.00
Placental		140,000	Liver	0.371	0.367	0.361	0.87	2.00
Amylase	Baboon	57,086	Kidney	27.9	0.734	0.722	0.87	2.00
Lipase	Baboon	48000	Kidney	27.9	0.734	0.722	0.87	2.00
Alanine Transaminase	Dog	54,600	Sinusoidal	0.949	0.530	0.535	1.2	2.71
Aspartate Aminotransferase	Dog
Mitochondrial		47,517	Kupffer	56.9	0.530	0.535	1.2	2.71
Cytoplasmic		46,247	Sinusoidal	4.06	0.530	0.535	1.2	2,71
Creatine kinase	Dog	81,000	Kupffer	31.7	0.370	0.374	1.2	2.71
Lactate Dehydrogenase	Lamb
LD1 Heart		138,000	Sinusoidal	0.975	0.53	0.53	3.85	1.35
LD5 Skeletal muscle		138,000	Kupffer	6.35	0.53	0.53	3.85	1.35