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CD spectra of GC-DNA in the presence of (A) spermine (100 μM to 10 mM), (B) protamine (2–15 μM), (C) Ni2+ (100 μM to 8 mM), and (D) cohex (50 μM to 3 mM) and of AT-DNA in the presence of (E) spermine (500 μM to 20 mM), (F) protamine (8–100 μM), (G) Ni2+ (2–10 mM), and (H) cohex (50 μM–3 mM). The concentration of each DNA was 50 μM in base pairs. Melting profiles of GC- and AT-DNA with condensing ligands.
