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. 2023 Jan 22;19(3):994–1006. doi: 10.7150/ijbs.76214

Figure 2.

Figure 2

Effects of PML knockdown or overexpression on cardiac fibroblasts. (A) The effects of si-PML on cell viabilities in CFs were examined by the CCK8 assay. (n = 4). (B-D) qRT-PCR analysis of Col-1α1, Col-3α1 and α-SMA mRNA levels in CFs transfected with si-PML (n = 4). (E) Analysis of the expression of α-SMA by western blot after transfection with si-PML (n = 4). (F) The effects of PML overexpression on cell viabilities in CFs were examined by the CCK8 assay (n = 4). (G-I) qRT-PCR analysis of Col-1α1, Col-3α1 and α-SMA mRNA levels in CFs transfected with PML plasmid (n = 4). (J) Analysis of the expression of α-SMA by western blot after transfection with PML plasmid (n = 4). (K) The effects of PML overexpression on cell viabilities in TGF-β1 treated CFs were examined by the CCK8 assay (n = 4). (L-N) qRT-PCR analysis of Col-1α1, Col-3α1 and α-SMA mRNA levels in CFs transfected with PML plasmid and treated with TGF-β1 (n = 4). (O) Western blot quantification of α-SMA expression (n = 4). *P < 0.05, **P < 0.01, ***P < 0.001.