RPMI complete medium (R10)
| Reagent | Final concentration | Amount |
|---|---|---|
| RPMI (Gibco 22400121) | 89% | 445 mL |
| FBS (R&D Systems S12550H) | 10% | 50 mL |
| Penicillin/Streptomycin (Gibco 15140122) | 1% | 5 mL |
| Total | N/A | 500 mL |
The media can be stored up to 3 months at 2°C–8°C.
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