35% ethanol
| Reagent | Final concentration | Amount |
|---|---|---|
| Ethanol 100% (CAS 64-17-5) | 35% | 33.94 mL |
| Distilled water | 65% | 66.05 mL |
| Total | N/A | 100 mL |
The solution must be prepared fresh to pre-wet the plate. Dilution is calculated according to Gay Lussac’s table.
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