Table 3. Calibration Equations, Correlation Coefficients, LOD and LOQ Values, and Working Concentration Ranges of the Protection of the A Base in dsDNA Depending on the Oxidative Damage Preventive Activities of Various AOs (n = 6).
| AO | working ranges (μM) | calibration equationsa | r | LODb (μM) | LOQc (μM) |
|---|---|---|---|---|---|
| QR | 5–50 | y = 0.421x + 11.14 | 0.995 | 1.0 | 3.0 |
| CT | 5–50 | y = 0.419x + 8.38 | 0.999 | 1.0 | 3.0 |
| RT | 5–50 | y = 0.387x + 10.46 | 0.994 | 1.0 | 3.0 |
| GA | 5–50 | y = 0.314x + 9.95 | 0.995 | 1.0 | 4.0 |
| ECG | 10–100 | y = 0.231x + 7.45 | 0.992 | 4.0 | 13.0 |
| EC | 5–50 | y = 0.209x + 8.21 | 0.999 | 2.0 | 6.0 |
| CA | 10–100 | y = 0.169x + 11.67 | 0.996 | 4.0 | 13.0 |
| NAC | 10–100 | y = 0.166x + 10.87 | 0.998 | 5.0 | 17.0 |
| AA | 25–250 | y = 0.064x + 11.63 | 0.999 | 4.0 | 14.0 |
| GSH | 10–250 | y = 0.059x + 12.93 | 0.998 | 4.0 | 13.0 |
| RA | 10–250 | y = 0.052x + 9.45 | 0.993 | 5.0 | 17.0 |
a
y = – ΔI (μA) and x = μmol/L
b
LOD = 3sbl/m (m: the slope of the calibration line and sbl: the standard deviation of the blank).
c
LOQ = 10sbl/m.