Fig. 7.

ApoE4 downregulates ACE2 protein expression in vivo. A ApoE-TR mice were selected to evaluate the regulatory effect of ApoE4 on ACE2 protein expression. B ACE2 protein levels in the heart, liver, lung, kidney, brain, and bowel (equivalent amounts of total proteins) of ApoE3-TR mice were measured by western blotting, n = 4 mice per group. C-I ACE2 protein levels in the heart, liver, cortex, hippocampus, bowel, kidney, and lung of ApoE2-TR, ApoE3-TR and ApoE4-TR mice were measured by western blotting. The results were normalized to the expression of a-tubulin. n = 6 mice per group. The data are expressed as the mean ± SD. Statistical differences were evaluated by one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001