Fig. 3.

Early-life starvation and IIS have similar effects on the adult lipid profile including extensive effects on abundance of phosphatidylcholine species. a) Pooled lipid abundance for each class of targeted lipids is plotted for the six conditions assayed. Open dots indicate sum of all species within each class for a biological replicate. Closed dots indicate the mean. b) Abundance of individual phosphatidylcholine species is plotted. Open dots indicate abundance in an individual biological replicate. Closed dots indicate the mean. a and b) Lipid levels normalized by total lipid. *P < 0.05, **P < 0.01, ***P < 0.001; lines connecting two conditions indicate significance in an unpaired t-test. Five pair-wise tests were considered here: control vs starved in each RNAi condition, EV vs daf-2 in starved worms, and daf-2 vs daf-16; daf-2 in starved worms. See Supplementary File 6 for complete statistical analysis.