Fig. 2.

Wnt signaling promotes starvation-induced gonad abnormalities. a) Frequency of all gonad abnormalities for worms starved 1 day (control) or 8 days (starved) as L1 larvae and recovered for 72 h on the indicated RNAi food is plotted. There were at least 40 worms per replicate. b) Frequency of all gonad abnormalities for control and starved worms of the indicated genotypes recovered for 72 h. There were at least 35 worms per replicate. c) Representative images of control and starved wild-type worms recovered for 90 h on bar-1 or pop-1 RNAi are presented. Images were taken at 200× total magnification. d) Representative images of control and starved gld-1(q485) mutant worms recovered for 90 h on empty vector, bar-1, or pop-1 RNAi are presented. Visible portions of the gonad are outlined with a dotted line. Images were taken at 400× total magnification. e) Frequency of large mitotic tumors for control and starved gld-1(q485) worms recovered for 90 h on empty vector, bar-1, or pop-1 RNAi are plotted. There were at least 12 worms per replicate; most replicates have >50 worms. a, b, and e) Circles represent biological replicates. Cross bars reflect the mean. *P < 0.05; **P < 0.01; ***P < 0.001; t-tests on means of replicates between empty vector RNAi and the indicated genotype or condition. c and d) Scale bars are 50 μm. The letter “v” indicates the location of the vulva. ev, empty vector.