Fig. 6.

Renal γδ T cells produce type-3 cytokines in response to inflammatory cytokines. TCRdH2BeGF mice were infected with 10⁷ CFU of S. aureus. After 4 wk, mice were treated with ampicillin for 2 wk. Leucocytes were isolated from the kidney and after T cell enrichment by magnetic negative selection, γδ T cells were isolated based on their GFP expression by flow cytometry. γδ T cells were stimulated either with cytokines (IL-1β, IL-6, and IL-23), anti-CD3, and anti-CD28 mAb, heat-killed S. aureus, or Pam₃Cys-Ser-(Lys)₄. After 72 h, cytokines were determined in the supernatant. Cells were stimulated and analyzed in triplicates or quadruplicates. One representative experiment out of two is shown. LOD: IL-17A 2.0 pg/ml, IFN-γ 1.7 pg/ml, TNF-α 1.5 pg/ml, IL-22 1.8 pg/ml, IL-17F 1.9 pg/ml, IL-10 1.8 pg/ml.