Figure 5.
Neurorestoration analysis – GdnfcHyper and WT mice injected with LC followed by AAV-Cre and AAV5-GDNF injection three weeks later. A, Schematic of experiment depicting unilateral injections of LC just above the SN and AAV-Cre or AAV5-GDNF into the STR three weeks later. B, Striatal Gdnf mRNA levels measured with qPCR at the endpoint (26W). One-way ANOVA, Tukey’s multiple comparisons test. p = 0.0203, p = 0.0001, p < 0.0001. C, Number of net contralateral turns (contralateral minus ipsilateral) in the elevated body swing test two weeks after LC injection (out of 20 turns). Unpaired t test. p = 0.0001. D, Number of contralateral turns (left-biased turns) in the elevated body swing test after LC and AAV-Cre injections. Two-way repeated measures ANOVA, Tukey’s multiple comparisons test. E, Latency to fall in the accelerating rotarod test. Two-way repeated measures ANOVA, Tukey’s multiple comparisons test. F, Time to touch the adhesive in the left forepaw in the adhesive removal test. Two-way repeated measures ANOVA, Tukey’s multiple comparisons test. G, Time to remove the adhesive from the left forepaw in the adhesive removal test. Two-way repeated measures ANOVA, Tukey’s multiple comparisons test. LC, lactacystin; cHyper, conditional hypermorphic; WT, wild type; STR, striatum; SN, substantia nigra; EBS, elevated body swing; RR, rotarod; ART, adhesive removal test. *p < 0.05, **p < 0.01, ***p < 0.001. see Extended Data Figure 5-1 for open field, home-cage activity, nesting score, and body weight data and Extended Data Figure 5-2 for IBA1 and GFAP immunohistochemistry data.