Fig. 6.

CLA supplementation alleviated LPC induced demyelination and neurological deficits. (A) Schematic depicting the experimental strategy for CLA supplementation in mice with a two-point LPC injection. (B) Cognitive function was evaluated using the Morris water maze. Representative images represent the swim paths. n = 8 mice per group. Data are expressed as mean ± SD, two-way repeated measure ANOVA for acquisition trial analysis, and unpaired t test for probe trial analysis; P = 0.0014, ***P < 0.001, ns, not significant vs. the vehicle group. (C) Representative images and quantification of LFB staining. n = 6 mice per group. Data are expressed as mean ± SD, unpaired t test; ****P < 0.0001 vs. the vehicle group. (Scale bar, 200 μm.) (D) Electron microscopy images of the corpus callosum with or without CLA supplementation at 28 dpi. Asterisks mark demyelinated axons. Quantification of g-ratios. n = 6 mice per group, n = 40 axons per mouse. Data are expressed as mean ± SD, unpaired t test; ****P < 0.0001 vs. the vehicle group. (Scale bar, 2 μm.) (E) Representative images and quantification of the corpus callosum stained for Olig2 (total oligodendrocytes), APC (mature oligodendrocytes), and BrdU (proliferative marker). n = 6 mice per group. Data are expressed as mean ± SD, One-way ANOVA with Bonferroni’s post hoc test; *P < 0.05, **P < 0.01. (Scale bar, 50 μm.) (F) Representative images of the corpus callosum stained for Iba-1 (microglia), Mac2 (phagocytosis), BODIPY (LD), and dMBP (myelin debris). Quantification of the BODIPY⁺ and dMBP⁺ areas. n = 6 mice per group. Data are expressed as mean ± SD, unpaired t test; ***P < 0.001 vs. the vehicle group. (Scale bars, 100 μm (Left) and 20 μm (Right).)