Fig 1.

Expression of phosphorylation sites in cultured tubular epithelial cells from WT and PFKFB2 KI mice ± metformin stimulation (A-C). The mutated phosphorylation site at PFK-FB2-Ser⁴⁸³ was not detectable in the PFKFB2 KI mice as expected (A). Expression of phospho-Ser⁴⁸³ was not significantly different with metformin stimulation (B). There was increased expression of phosphorylated ACC-Ser⁷⁹ phosphorylation with metformin stimulation in both WT and PFKFB2 KI cells (A, C ***p<0.001 and *p = 0.01 respectively). Expression of phosphorylated ACC-Ser⁷⁹ was decreased in PFKFB2 KI metformin cells compared to WT cells (A, C *p = 0.02). Mean + SD.