Fig 5.

Measurement of markers of inflammation via RT-PCR in WT and PFKFB2 KI kidneys subjected to UUO ± metformin (A-D). mRNA expression of F4.80, TNF-α, Interleukin-1 (IL-1) and Interleukin-6 (IL-6) was significantly reduced with the addition of metformin in wild-type mice (A-D ***p = 0.0001, **p = 0.0016, *p = 0.0230, **p = 0.0008 respectively). In the PFKFB2 KI mice, there was decreased mRNA expression of F4.80 and TNF-α with the addition of metformin (A, B **p = 0.0024, *p = 0.0277 respectively). There was no significance difference between WT and PFKFB2 KI mouse groups in any of these parameters measured. Mean + SD.