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. 2023 Feb 9;21(2):e3001967. doi: 10.1371/journal.pbio.3001967

Fig 4. LRRC15 is expressed in lung fibroblasts and lines the airways in COVID-19 patients.

Fig 4

(A) Overview of cell types expressing LRRC15 from existing single-cell RNA-sequencing datasets. (B) UMAP plot of lung single-nucleus RNA seq dataset (Melms and colleagues). (C) Feature plot and (D) dotplot shows LRRC15 is expressed in fibroblasts and neuronal cells. Expression of LRRC15 is also observed in fibroblasts of separate studies (See S5 Fig). (E) Proportion of cells that are lung fibroblasts increases with COVID lungs (7.9% in control (N = 19) and 22.9% in COVID (N = 47); unpaired t test, p < 0.0001). (F and G) Representative micrograph of HE-stained lung tissue section obtained from (F) a human donor without COVID-19 and (G) donor diagnosed with COVID-19. Imaging performed at 200× magnification (scale bar = 50 m). All images in S6A Fig (Control, N = 1; COVID-19, N = 4). (H and I) Representative micrograph of immunofluorescence staining in human lung tissue section obtained from (H) donor without COVID-19 and (I) donor diagnosed with COVID-19. Images were taken at 200× magnification (scale bar = 100 m). Red = Collagen I, green = LRRC15, blue = DAPI. All images in S6B Fig (Control, N = 3; COVID-19, N = 4). The data underlying all panels in this figure can be found in DOI: 10.5281/zenodo.7416876. ACE2, angiotensin-converting enzyme 2; COVID-19, Coronavirus Disease 2019; HE, hematoxylin and eosin; LRRC15, leucine-rich repeat-containing protein 15.