Fig. 4.

Effects of rapamycin and Pde1 overexpression on N1199. a) Galactose-regulated PDE1 was overexpressed in YJM1199 under Gal control and 2 independent colonies were analyzed (1,2). Empty vec. indicates vector control with galactose induction. Un-uninduced PDE1. N1199-cured isolates were tested phenotypically.1-YSV860, 2-YSV861 (Supplementary Table 1). b) YJM1199 cultures were incubated with 0–100 nM rapamycin for 4 days, allowed to recover on YPD, and checked for the presence/absence of N1199. Strains cured of N1199 at 5 different concentrations were tested phenotypically (1,199 Rapa 0.1–100nM-treated, YSV869-873, respectively).