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Primers used for PCR detection.
| Gene | Primers | Primer's sequences (5′-3′) | Annealing temperature (°C) | Amplicon size | References |
|---|---|---|---|---|---|
| B646L | P72-U | F: GGCACAAGTTCGGACATGT | 55 | 478 bp | [4] |
| P72-D | R: GTACTGTAACGCAGCACAG | ||||
|
| |||||
| EP402R | ASF_CD2v_ga3611-F | F: TATAATATAACAAATAATTGTAG | 55 | 850 bp | 15 |
| ASF_CD2v_ga4220-R | R: AGGGACGCATGTAGTAAATAG | ||||
|
| |||||
| IGR | ASF_IGR_I73R_F | F: TGTCGTCTTACCTACAGGAT | 58 | 651 bp | [14] |
| ASF_IGR_I73R_R | R: TTCATATGCTTGTTGCGTTC | ||||
