Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 2;7(2):264–278. doi: 10.1038/s41559-022-01925-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 7 — a, Multiple alignment of the CDS region of ENSG00000205704 in human with the orthologous regions in chimpanzee, rhesus macaque and mouse. The protein sequence encoded by ENSG00000205704 in human was shown, with the start and stop codons highlighted in the diagram of the gene. b, The design of CRISPR/Cas9 assay to knock-out ENSG00000205704, with the sequences and the target regions of the gRNAs shown. The ENSG00000205704-KO hESC line was further verified by Sanger sequencing, as shown below the diagram. c, Immunofluorescence staining of HA tag (HA, indicative of the expression of ENSG00000205704 protein with HA tag) and OCT4 (OCT4) in hESCs with the overexpression of ENSG00000205704 (OE). Scale bars, 20 μm. This experiment was repeated 5 times independently with similar results. d, PCR validation of the knock-out assay. CTRL, wild type hESCs; ENSG00000205704-KO, three replicates of ENSG00000205704-KO hESCs with the target region removed on both chromosomes. e, Western blots showing the expression of the HA-tagged ENSG00000205704 protein in wide type hESCs (CTRL), and the hESCs with the over expression of the HA-tagged ENSG00000205704 protein (OE), This experiment was repeated 3 times independently with similar results.