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. 2023 Jan 2;7(2):264–278. doi: 10.1038/s41559-022-01925-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — a, Left panel, Immunofluorescence staining of marker genes of hESC pluripotency for wild type hESCs (CTRL) and hESCs with ENSG00000205704 knock-out (KO). Right panel, quantifications of the immunofluorescence staining. Scale bars, 100 μm; n = 6 biologically independent clones; data are presented as mean values + /- SEM. b, Left panel, Immunofluorescence staining of marker genes of hESC pluripotency for wild type hESCs (CTRL) and hESCs with ENSG00000205704 over expression (OE). Right panel, quantifications of the immunofluorescence staining. Scale bars, 100 μm; n = 4 biologically independent clones; data are presented as mean values + /- SEM; two-sided, unpaired t-test. N.S, not significant.