Key resources table.

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody Alexa Fluor 680	Invitrogen	A-21057
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody Alexa Fluor 680	Invitrogen	A-11011
Anti-SarZ mouse polyclonal sera	This paper	N/A
Anti-ClfB rabbit polyclonal sera	Fleury et al. 201779	N/A
Bacterial and virus strains
AH-LAC	Boles et al. 201019	N/A
FPR3757	Isolated from a patient (Diep et al. 2006)20	N/A
SF8300	Isolated from a patient (Diep et al. 2008)21	N/A
AH-LAC Δluk	Blake et al. 201859	N/A
AH-LAC sarZ::bursa	This paper	N/A
AH-LAC + vector	This paper	N/A
AH-LAC sarZ::bursa + vector	This paper	N/A
AH-LAC sarZ::bursa + sarZWT	This paper	N/A
AH-LAC sarZ::bursa + sarZNS1	This paper	N/A
AH-LAC sarZ::bursa + sarZNS2	This paper	N/A
AH-LAC sarZ::bursa + sarZFS1	This paper	N/A
AH-LAC sarZ::bursa + sarZFS2	This paper	N/A
I1 sarZ::tet	This paper	N/A
I1 sarZ::sarZWT	This paper	N/A
H2 sarZ::tet	This paper	N/A
H2 sarZ::sarZWT	This paper	N/A
H2 sarZ::tet + vector	This paper	N/A
H2 sarZ::tet + sarZWT	This paper	N/A
H2 sarZ::tet + sarZFS1	This paper	N/A
SF8300 sarZ::tet	This paper	N/A
I2 clfB::tet	This paper	N/A
I2 clfB::clfB	This paper	N/A
H2 clfB::tet	This paper	N/A
BL21 pET15b-sarZ	This paper	N/A
BL21 pET15b-sarZNS1	This paper	N/A
BL21 pET15b-sarZNS2	This paper	N/A
Clinical isolates listed in Table S2	This paper	N/A
Biological samples
LeukoPacks	New York Blood Center	N/A
SeraCare Normal Human Serum	Dba LGC Clinical Diagnostics	Ref# 27000-100
Whole human blood	Torres Lab IRB	IRB # S12-01223
Chemicals, peptides, and recombinant proteins
Lysostaphin	Smbi Products LLC	Cat# LSPN-50
DNase (for bacterial whole cell lysates)	Promega	Cat# M6101
RNase A	Promega	Cat# A7973
HALT™ Protease Inhibitor	Thermo Scientific	Cat# 78438
Sodium pyruvate	ACROS Organics	Cat# 113-24-6
Chloramphenicol	Fisher BioReagents	Cat# BP904-100
Erythromycin	Fisher BioReagents	Cat# BP920-25
Tetracycline	Alfa Aesar	Cat# B21408
Ampicillin	Fisher Scientific	Cat# BP1760
Anhydrotetracycline	Thermo Scientific	Cat# AC233131000
PCPA	Sigma-Aldrich	Cat# C6506
IPTG	Research Products International	Cat# I5600025.0
TiterMax® Gold	Titermax	Cat# G-20
Avertin (2,2,2-tribromoethanol)	Sigma-Aldrich	Cat# T48402
Streptokinase	Sigma-Aldrich	Cat# S313-250KU4
Trypsin	Sigma-Aldrich	Cat# T4549
DNase I (used in SK buffer)	Sigma-Aldrich	Cat# DN25-1G
RNase (used in SK buffer)	Alfa Aesar	Cat# J61996
Dynabeads M-280 Streptavidin	Invitrogen	Cat# 11205D
Poly(dG:dC) naked	Invivogen	Cat# TLRLPGCN
His-SarZWT	This paper	N/A
His-SarZNS1	This paper	N/A
His-SarZNS2	This paper	N/A
Critical commercial assays
CellTiter 96® Aqueous One Solution	Promega	Cat# PR-G3581
CytoTox-ONE™ Homogeneous Membrane Integrity Assay	Promega	Cat# PR-G7892
RNA-STAT-60™	Amsbio	Cat# CS-111
TURBO DNA-free™ kit	Invitrogen	Cat# AM1907
Ribo-Zero Plus rRNA Depletion Kit	Illumina	Cat# 20037135
Stranded Total RNA Prep kit	Illumina	Cat# AM1907
Gibson Assembly Master Mix	New England Biolabs	Cat# E2611L
DNeasy Blood and Tissue Kit	Qiagen	Cat# 69504
Bugbuster	MilliporeSigma	Cat# 709215
HisTrap HP column	Cytiva	Cat# 45-003-013
SuperScript™ III First-Strand Synthesis System	Invitrogen	Cat# 1808004
Deposited data
Genome assemblies	GenBank	See Table S2
mRNA sequence files	NCBI Gene Expression Omnibus	Accession No. GSE185544
Experimental models: Cell lines
NA
Experimental models: Organisms/strains
Swiss Webster Hsd (5 week old)	Envigo Inc	Hsd:ND4
C57BL/6J (8 week old)	Jackson Laboratories	000664
Oligonucleotides
For primers used in cloning, see Table S4	This paper	N/A
For primers used in qRT-PCR, see Table S4	This paper	N/A
For primers used in promoter pulldowns, see Table S4	This paper	N/A
Recombinant DNA
pOS1	Schneedwind et al. 199244	N/A
pIMAY	Monk et al. 201260	Addgene plasmid #68939
pIMAY*	Schuster et al. 201961	Addgene plasmid #121441
pET15b	Novagen	Cat# 69661-3
Software and algorithms
HGAP3	Chin et al. 201363	https://www.pacb.com/products-and-services/analytical-software/smrt-analysis/
Parsnp	Treangen et al. 201464	http://github.com/marbl/harvest
etetoolkit	Huerta-Cepas et al. 201665	http://etetoolkit.org/
M.viridis	This paper	10.5281/zenodo.7159034
Nucdiff	Khelic et al. 201766	https://github.com/uio-cels/NucDiff
GWviz	This paper	10.5281/zenodo.7159034
Roary	Page et al. 201567	https://sanger-pathogens.github.io/Roary/
operon-mapper	Taboada et al. 201868	https://biocomputo.ibt.unam.mx/operon_mapper/
Pyseer	Lees et al. 201870	https://github.com/mgalardini/pyseer
NCBI datasets	Sayers et al. 202272	https://www.ncbi.nlm.nih.gov/datasets/
matplotlib	Hunter et al. 201775	https://matplotlib.org/
MLST	Seeman (Unpublished)	https://github.com/tseemann/mlst
cgMLST	Leopold et al. 201477	https://www.cgmlst.org
Grapetree	Zhou et al. 201878	https://github.com/achtman-lab/GrapeTree
PubMLST	Jolley et al. 201073	https://pubmlst.org/
cutadapt	Martin 201183	https://github.com/marcelm/cutadapt/
bowtie2	Langmead et al. 201284	https://bowtie-bio.sourceforge.net/bowtie2/index.shtml
htseq-count	Anders et al. 201585	https://github.com/htseq/htseq
bioconductor	Huber et al. 201587	https://bioconductor.org
limma	Ritchie et al. 201586	https://bioconductor.org/packages/release/bioc/html/limma.html
Prism	GraphPad Inc	https://www.graphpad.com/scientific-software/prism/
Other