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. 2023 Feb 9;13:2304. doi: 10.1038/s41598-023-29423-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Blocking CD36 significantly alters TLR-2 and TLR-3 signalling. (a) Western blot of whole brain protein lysates from the control (saline), anti-CD36, LPS and anti-CD36 + LPS injected pups. (b) Iba1, (c) TLR2, (d) TLR3, (e) IRF7, (f) phosrpho-P65 expression levels were quantified in different conditions. GAPDH was used as loading control A significant increase in the levels of all the tested proteins is observed after LPS injection. Treating the pups with anti-CD36 before LPS injections considerably reduces their expression to the level as observed in the control pups. (g) Western blot of total protein lysates from the control, anti-CD36, LPS and anti-CD36 + LPS injected mice. (h) TLR4, (i) IRF3, (j) iNOS expression levels were quantified in above-described conditions. GAPDH is used as loading control. A significant increase in the levels of all the proteins tested is observed after LPS injection when compared to control and anti-CD36 treated mice. Of note, LPS-mediated increase in expression of TLR4/IRF3 is maintained in the anti-CD36 + LPS treated group. The entire data was presented as mean ± SEM and statistical significance between the groups was achieved using one-way ANOVA with Tukey’s multiple comparison test (ctl vs LPS, ctl vs anti-CD36, LPS vs anti-CD36 + LPS) and depicted as ****p ≤ 0.0001; ***p ≤ 0.001; **p ≤ 0.01 and *p ≤ 0.05.