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Differential expression of S1PRs in pancreatic cancer cells. mRNA levels of S1PR1, S1PR3, S1PR4 and S1PR5 relative to S1PR2 (designated =1) are shown. a. Murine Panc02-luc and AsPC-1 cells predominantly express S1PR2. b. Human MIA PaCa2 and BxPC-3 express higher levels of S1PR-5. mRNA levels of individual S1PRs were detected by real-time RTPCR and normalized using GAPDH as an internal control. *P<0.001, compared to S1PR2; n=6.
