Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 10;14:737. doi: 10.1038/s41467-023-36403-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a, b Influence of dsLsMLP treatment on the LsSP1 abundance in salivary gland quantified by qRT-PCR (a) and western-blotting (b) assays. Data are presented as mean values ±SEM (n = 3 independent biological replicates). P-values were determined by two-tailed unpaired Student’s t test. ns, not significant. c LsSP1 staining of salivary sheath secreted from dsRNA-treated Laodelphax striatellus. The salivary sheaths secreted from dsGFP- or dsLsMLP-treated L. striatellus were probed with the anti-LsSP1 serum conjugated with Alexa Fluor™ 488 NHS Ester (green), and examined by Leica SP8. Bars = 20 μm. d Detection of LsSP1 in watery (lane1, lane3) and gel saliva (lane 2, lane 4) secreted from dsGFP- or dsLsMLP-treated L. striatellus. e Yeast two hybrid assays showing the interaction between LsSP1 and LsMLP. The different combinations of constructs transformed into yeast cells were grown on the selective medium SD/-Trp/-Leu (DDO), and the interactions were tested with SD/-Trp/-Leu/-His/-Ade (QDO). f Bimolecular fluorescence complementation (BiFC) assays showing the interaction between LsSP1 and LsMLP. Bars = 20 μm. g The co-expression scheme in Nicotiana benthamiana leaves during Luciferase complementation (LUC) assays. h Results from LUC assays showing the interaction between LsSP1 and LsMLP. Bar = 1 cm. Experiments in b, c, d, f, and h were repeated three times with the similar results. Source data are provided as a Source Data file.