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. 2023 Jan 27;14:1089809. doi: 10.3389/fimmu.2023.1089809

Figure 3.

Figure 3

BRG1 is the potential target gene of miR-222-3p and inhibition of miR-222-3p promotes the activation of Nrf2/HO-1 signaling. (A) The alignment of miR-222-3p with the seed regions in BRG1 3′-UTR. (B) Designed wild-type or mutant sequences of BRG1 binding to miR-222-3p, and relative luciferase activity was detected by dual-luciferase reporter assay. (C) Relative BRG1 mRNA expression was determined by qRT-PCR from NCM460 cells. (D, E) Relative BRG1 protein expression was determined by Western blot from NCM460 cells. (F) Relative Nrf2 mRNA expression was determined by qRT-PCR from NCM460 cells. (G, H) Relative Nu-Nrf2 protein expression was determined by Western blot from NCM460 cells. (I) Relative HO-1 mRNA expression was determined by qRT-PCR from NCM460 cells. (J, K) Relative HO-1 protein expression was determined by Western blot from NCM460 cells. Data are presented as the mean ± SD (n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001. Control: control group; DSS+miR-NC: DSS+negative control miRNA group; DSS+miR-222-3p mimics: miR-222-3p mimics group; DSS+miR-222-3p inhibitor: miR-222-3p inhibitor group. Scale bar: 200 µm.